Journal: The Korean Journal of Internal Medicine

Article Title: Harmonization of laboratory results by data adjustment in multicenter clinical trials

doi: 10.3904/kjim.2017.034

Figure Lengend Snippet: Comparison data of the six analytes

Article Snippet: In its 2015 survey, the College of American Pathologists (CAP), one of the largest external quality assessment organizations in the world, showed that the inter-assay coefficient of variation (CV) of total cholesterol was 3.3% across all methods, 3.9% for creatinine, and 3.2% for hemoglobin A1c [ ].

Techniques: Comparison

Journal: The Korean Journal of Internal Medicine

Article Title: Harmonization of laboratory results by data adjustment in multicenter clinical trials

doi: 10.3904/kjim.2017.034

Figure Lengend Snippet: The comparison of inter-assay coefficient of variation (CV) before and after adjustment (CVadj). With the data adjusted based on Deming regression analyses between the central laboratory and each laboratory, mean CVs of all analytes decreased. HDL-C, high density lipoprotein cholesterol; LDL-C, low density lipoprotein cholesterol.

Article Snippet: In its 2015 survey, the College of American Pathologists (CAP), one of the largest external quality assessment organizations in the world, showed that the inter-assay coefficient of variation (CV) of total cholesterol was 3.3% across all methods, 3.9% for creatinine, and 3.2% for hemoglobin A1c [ ].

Techniques: Comparison, Inter Assay

Journal: The Korean Journal of Internal Medicine

Article Title: Harmonization of laboratory results by data adjustment in multicenter clinical trials

doi: 10.3904/kjim.2017.034

Figure Lengend Snippet: Comparison of CV (before and after adjustment) with reference criteria

Article Snippet: In its 2015 survey, the College of American Pathologists (CAP), one of the largest external quality assessment organizations in the world, showed that the inter-assay coefficient of variation (CV) of total cholesterol was 3.3% across all methods, 3.9% for creatinine, and 3.2% for hemoglobin A1c [ ].

Techniques: Comparison

Journal: The Korean Journal of Internal Medicine

Article Title: Harmonization of laboratory results by data adjustment in multicenter clinical trials

doi: 10.3904/kjim.2017.034

Figure Lengend Snippet: CV before and after adjustment by deming regression

Article Snippet: In its 2015 survey, the College of American Pathologists (CAP), one of the largest external quality assessment organizations in the world, showed that the inter-assay coefficient of variation (CV) of total cholesterol was 3.3% across all methods, 3.9% for creatinine, and 3.2% for hemoglobin A1c [ ].

Techniques:

Journal: bioRxiv

Article Title: Total testosterone is not associated with muscle mass, function or exercise adaptations in pre-menopausal females

doi: 10.1101/2024.05.12.593786

Figure Lengend Snippet: Hormone levels across 12 weeks of resistance training. (A) Total testosterone (TT), (B) sex hormone binding globulin (SHBG) and (C) free androgen index (FAI), (D) DHEA, (E) DHT concentrations. Participants undertaking a 12-week gym-based training program are denoted in blue (n=16), and participants undertaking the blended resistance training program are denoted in red (n=10).

Article Snippet: Testosterone (sensitivity 0.18 ng·mL -1 , intra-assay coefficient of variation (CV) 3.1-5.4%, inter-assay CV 4.2-7.4%), sex hormone binding globulin (SHBG; sensitivity 0.23 nmol·L -1 , intra-assay CV 2.3-4.8%, inter-assay CV 5.2-6.3%), dehydroepiandrosterone (DHEA; sensitivity 0.03 ng·mL -1 , intra-assay CV 3.9-7.6%, inter-assay CV 5.1-10.4%) and 5α-dihydrotestosterone (DHT; sensitivity 7.23 pg·mL -1 , intra-assay CV 3.33-6.25%, inter-assay CV 6.49-7.47%) were measured via Enzyme-Linked Immunosorbant Assay (ELISA; #IBRE52151, #IB30176808, #IBRE52221, #IBDB5202, Abacus Dx, Parkville, Australia), according to manufacturer’s instructions.

Techniques: Binding Assay

Journal: bioRxiv

Article Title: Total testosterone is not associated with muscle mass, function or exercise adaptations in pre-menopausal females

doi: 10.1101/2024.05.12.593786

Figure Lengend Snippet: Twelve weeks of resistance training did not affect concentrations of (A) total testosterone (nmol·L -1 ), (B) the free androgen index (FAI; AU), (C) dihydrotestosterone (DHT; pg·mL -1 ) or (D) dehydroepiandrosterone (DHEA; nmol·L -1 ) in plasma of previously untrained, pre-menopausal females (n=27). Data were analysed using a one-way ANOVA. Values are represented as mean ± SD.

Article Snippet: Testosterone (sensitivity 0.18 ng·mL -1 , intra-assay coefficient of variation (CV) 3.1-5.4%, inter-assay CV 4.2-7.4%), sex hormone binding globulin (SHBG; sensitivity 0.23 nmol·L -1 , intra-assay CV 2.3-4.8%, inter-assay CV 5.2-6.3%), dehydroepiandrosterone (DHEA; sensitivity 0.03 ng·mL -1 , intra-assay CV 3.9-7.6%, inter-assay CV 5.1-10.4%) and 5α-dihydrotestosterone (DHT; sensitivity 7.23 pg·mL -1 , intra-assay CV 3.33-6.25%, inter-assay CV 6.49-7.47%) were measured via Enzyme-Linked Immunosorbant Assay (ELISA; #IBRE52151, #IB30176808, #IBRE52221, #IBDB5202, Abacus Dx, Parkville, Australia), according to manufacturer’s instructions.

Techniques:

Journal: bioRxiv

Article Title: Total testosterone is not associated with muscle mass, function or exercise adaptations in pre-menopausal females

doi: 10.1101/2024.05.12.593786

Figure Lengend Snippet: The androgen profile measured by LC/MS from urine in pre-menopausal females is not influenced by resistance training. The concentrations of a) testosterone (nmol·L -1 ), b) epitestosterone (nmol·L -1 ), c) androsterone (nmol·L -1 ), d) etiocholanolone (ng·mL -1 ), e) 5α-adiol (ng·mL -1 ), f) 5β-adiol (ng·mL -1 ), g) DHEA (nmol·L -1 ) and h) DHT (nmol·L -1 ) were stable across twelve weeks of resistance training in our cohort (n=27). Data were analysed with one-way ANOVA. Bars indicate mean ± SD.

Article Snippet: Testosterone (sensitivity 0.18 ng·mL -1 , intra-assay coefficient of variation (CV) 3.1-5.4%, inter-assay CV 4.2-7.4%), sex hormone binding globulin (SHBG; sensitivity 0.23 nmol·L -1 , intra-assay CV 2.3-4.8%, inter-assay CV 5.2-6.3%), dehydroepiandrosterone (DHEA; sensitivity 0.03 ng·mL -1 , intra-assay CV 3.9-7.6%, inter-assay CV 5.1-10.4%) and 5α-dihydrotestosterone (DHT; sensitivity 7.23 pg·mL -1 , intra-assay CV 3.33-6.25%, inter-assay CV 6.49-7.47%) were measured via Enzyme-Linked Immunosorbant Assay (ELISA; #IBRE52151, #IB30176808, #IBRE52221, #IBDB5202, Abacus Dx, Parkville, Australia), according to manufacturer’s instructions.

Techniques: Liquid Chromatography with Mass Spectroscopy

Journal: bioRxiv

Article Title: Total testosterone is not associated with muscle mass, function or exercise adaptations in pre-menopausal females

doi: 10.1101/2024.05.12.593786

Figure Lengend Snippet: Testosterone treatment over 7 days of differentiation increased (A) myotube diameter, (B) AR protein content and (C) AR intensity relative to the proportion of the visual field occupied by myocytes. AR cellular localisation in primary muscle cell lines treated with vehicle (CON) or 100 nM testosterone (TT) after D) 1 day or E) 7 days of treatment (n=6 female donors). Phalloidin (stains actin) appears in blue. AR appears in green. Scale is 0.25 µm·pixel -1 for all images. White scale bar represents 50 µm. Data were analysed using two-tailed, paired t-tests and two-way ANOVA. Values are represented mean ± SD. **indicates p<0.01.

Article Snippet: Testosterone (sensitivity 0.18 ng·mL -1 , intra-assay coefficient of variation (CV) 3.1-5.4%, inter-assay CV 4.2-7.4%), sex hormone binding globulin (SHBG; sensitivity 0.23 nmol·L -1 , intra-assay CV 2.3-4.8%, inter-assay CV 5.2-6.3%), dehydroepiandrosterone (DHEA; sensitivity 0.03 ng·mL -1 , intra-assay CV 3.9-7.6%, inter-assay CV 5.1-10.4%) and 5α-dihydrotestosterone (DHT; sensitivity 7.23 pg·mL -1 , intra-assay CV 3.33-6.25%, inter-assay CV 6.49-7.47%) were measured via Enzyme-Linked Immunosorbant Assay (ELISA; #IBRE52151, #IB30176808, #IBRE52221, #IBDB5202, Abacus Dx, Parkville, Australia), according to manufacturer’s instructions.

Techniques: Two Tailed Test

Journal: bioRxiv

Article Title: Total testosterone is not associated with muscle mass, function or exercise adaptations in pre-menopausal females

doi: 10.1101/2024.05.12.593786

Figure Lengend Snippet: Treatment with 100 nM testosterone did not significantly change the expression of A) Akt, B) p-Akt, C) mTOR, D) p-mTOR or E) p-MAPK compared to a vehicle control across 1, 4 or 7 days of differentiation. Black circles represent vehicle control condition (CON), white circles represent testosterone treated (TT) condition. Data were analysed via two-way, repeated measures ANOVA. Bars represent mean ± SD.

Article Snippet: Testosterone (sensitivity 0.18 ng·mL -1 , intra-assay coefficient of variation (CV) 3.1-5.4%, inter-assay CV 4.2-7.4%), sex hormone binding globulin (SHBG; sensitivity 0.23 nmol·L -1 , intra-assay CV 2.3-4.8%, inter-assay CV 5.2-6.3%), dehydroepiandrosterone (DHEA; sensitivity 0.03 ng·mL -1 , intra-assay CV 3.9-7.6%, inter-assay CV 5.1-10.4%) and 5α-dihydrotestosterone (DHT; sensitivity 7.23 pg·mL -1 , intra-assay CV 3.33-6.25%, inter-assay CV 6.49-7.47%) were measured via Enzyme-Linked Immunosorbant Assay (ELISA; #IBRE52151, #IB30176808, #IBRE52221, #IBDB5202, Abacus Dx, Parkville, Australia), according to manufacturer’s instructions.

Techniques: Expressing